Easy to perform and score, Validated reagent kit for Sperm DNA
fragmentation test (SCD method)
1. The kit is designed to evaluate the DNA intactness in human
sperm.
2. The assay is to provide medical evidence for the etiology of
infertility, the prediction of assisted reproductive pregnancy
outcome
and abortion.
3. The reagent kit has excellent staining for sperm, easy to
identify the fragmented sperm. The head halo of non-fragmented
sperm is
large, and the boundary between the head and halo is obvious.
4. Equipped with Specimen SCD preservation, fresh semen could be
stored for up to half a month and tested in batch. Easy for
storage and transportation.
5. The required device is general microscope and 80℃ water bath.
Without the need for complex laboratory equipment.
6. With wide range of users.
【Test principle】
Sperm Chromatin Dispersion (SCD) method: The basis of the test lies
in the differential response offered by the nuclei of spermatozoa
with fragmented DNA compared to those with their DNA intactness.
The controlled denaturation of the DNA followed by the extraction
of the nuclear proteins, gives rise to partially deproteinized
nucleoids in which the DNA loops expand, forming halos of chromatin
dispersion. However, the spermatozoa nucleoids whose DNA is
fragmented, either do not develop a dispersion halo or the halo is
minimal.
【Reference value】
*The normal reference value: the percentage of sperm with
fragmented DNA < 25%.
The identification of DNA fragmented sperm: the head of sperm only
produce small halos or not, and the thickness of the halos
in one side is less than 1/3 of the smallest diameter of the head
of sperm
